What to Consider While Choosing a Correct Vial for Chromatography?
Chromatography refers to the technique of separating, identifying, and purifying a mixture’s solutes or components for quantitative and qualitative analysis. Chromatography vials, generally made of polypropylene (PP), polymethylpentene, or silica, act as interim sample containers for liquid or gas chromatography analysis.
Choosing the correct vial and a chromatography lab vial supplier is essential for the samples and the staff’s security. The first step to selecting an ideal chromatography vial is to know and understand how to choose one or what factors to consider while choosing one. So, let’s begin!
How to Choose the Correct Chromatography Vial?
Decide on the Vial Closure Style
Screw thread vials do not need external tools to attach or remove the cap and are generally used for LCMS and HPLC analysis. On the contrary, crimp vials are generally used for gas chromatography analysis as GC vials. They are known to be the gold standard for sample evaporation.
Additionally, these vials are specially used where tamper-proof closures and chain of custody are required. Snap top vials are popular among lab technicians and are, at times, chosen over screw thread vials.
Choose the Vial Material
Vials made of plastic and glass are amongst the most popular. When it comes to choosing glass vials, know that their components are free from any impurities like metal that can potentially influence or hinder the experiment. Additionally, these vials can resist heat very well, which is essential because the chromatography process may require heating glass vials over 500 degrees Celsius.
Coming to plastic vials, please note that their use depends on the type of plastic used in the vial, which is generally either PP or PMP. PP vials are translucent and can resist heat of up to 135 degrees Celsius. At the same time, PMP vials are also translucent and can withstand heat of up to 175 degrees Celsius, ultimately increasing the sample’s visibility. Lastly, plastic vials are affordable and durable, which adds to their popularity.
Determine the Vial’s Color
When we talk about outer appearance, i.e., the color of the chromatography vials, you have the option to choose either from clear or amber in both glass and plastic vials. The color choice is important to ensure the sample stays protected from UV light, as it can influence some chemicals within the sample.
Determine Size
Chromatography vials are available in different sizes to suit several needs. Standard sizes generally include 2ml (12x32mm), 4ml (15x45mm), and 1ml (8x40mm). Based on their requirements, buyers can also choose from a 4mm or 6mm cap. While choosing, please consider whether your vials will be handled by a R.A.M. (Robotic Arm Machine) or not.
Wrapping Up
Choosing an ideal chromatography vial is important for accurate results. While there are a number of factors influencing the choice, it ultimately depends on the specific needs and applications. Last but not least, don’t forget to source it from a trusted chromatography vials supplier in USA.
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